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基础医学系

jijiakui

纪家葵

履历

    教育经历:

    1997/09-2003/09,美国康奈尔大学医学院/史隆凯特林癌症研究中心分子、细胞生物与遗传学系,博士

    1992/09-1997/08, 美国爱荷华州立大学,生物化学专业,学士和硕士

    工作经历:

    2010/05-至今, 清华大学,医学院,研究员、博士生导师

    2007/09-2010/04, 美国斯坦福大学,副研究员

    2004/05-2007/08, 美国加州大学旧金山分校,博士后研究

    2003/5-2004/05, 美国康奈尔大学,医学院/史隆凯特林癌症研究中心分子、细胞生物与遗传学系,博士后研究


研究领域与方向

生殖细胞传递生物的全能性及创造生物的多样性。精卵结合后,新生命跟据遗传图谱及表观遗传信息,逐渐发育生长。本实验室致力于研究生殖发育过程的分子机制,尤其是人类生殖细胞的形成、减数分裂的启动、生殖细胞对早期胚胎发育的影响,包括导致不孕不育症的遗传及分子机理研究。受限于取样的困难,我们对人类配子的形成与成熟过程的了解,多借助于模式生物,而模式生物与人类配子形成的机制有许多不同。因此,我们利用人类胚胎干细胞来构建生殖细胞体外分化发育的模型,这提供我们崭新的体外平台来研究人类生殖发育特有的机制。此外,我们也致力于研发治疗遗传疾病的分子及细胞生物学方法

〔1〕人类干细胞及生殖细胞的分化机制: 利用人类干细胞,结合分子生物学及细胞生物学,研究生殖细胞的分化机理,建立体外分化精子、卵子的系统平台

〔2〕人类遗传疾病的研究与治疗: 确定遗传突变的致病机理,结合干细胞及定点基因重组技术, 研发治疗遗传疾病的途径




学术荣誉与奖励

关键词

干细胞、生殖发育、遗传疾病

代表性论文

1.Jung, D., J. Xiong, M. Ye, X. Qin, L. Li, S. Cheng, M. Luo, J. Peng, J. Dong, F. Tang, W. Shen, M. M. Matzuk and K. Kee* (2017). "In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells." Nat Commun 8: 15680.

2.Zhu, C., Y. Gao, H. Guo, B. Xia, J. Song, X. Wu, H. Zeng, K. Kee, F. Tang* and C. Yi* (2017). "Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution." Cell Stem Cell 20(5): 720-731 e725.

3. Li, L., Y. Sha, X. Wang, P. Li, J. Wang, K. Kee and B. Wang* (2017). "Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa." Oncotarget 8(12): 19914-19922.

4.Guo, J., D. Ma, R. Huang, J. Ming, M. Ye, K. Kee, Z. Xie and J. Na* (2017). "An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells." Protein Cell. 8(5):379-393.

5.Li, L., B. Wang, W. Zhang, B. Chen, M. Luo, J. Wang, X. Wang, Y. Cao* and K. Kee* (2017). "A homozygous NOBOX truncating variant causes defective transcriptional activation and leads to primary ovarian insufficiency." Hum Reprod 32(1): 248-255.

6.He, Z. and K. Kee* (2017). "Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells." Methods Mol Biol 1498: 153-162.

7.Zhang, B., H. Zheng, B. Huang, W. Li, Y. Xiang, X. Peng, J. Ming, X. Wu, Y. Zhang, Q. Xu, W. Liu, X. Kou, Y. Zhao, W. He, C. Li, B. Chen, Y. Li, Q. Wang, J. Ma, Q. Yin, K. Kee, A. Meng, S. Gao, F. Xu, J. Na* and W. Xie* (2016). "Allelic reprogramming of the histone modification H3K4me3 in early mammalian development." Nature 537(7621): 553-557.

8.Li, L., X. Zhou, X. Wang, J. Wang, W. Zhang, B. Wang, Y. Cao* and K. Kee* (2016). "A dominant negative mutation at the ATP binding domain of AMHR2 is associated with a defective anti-Mullerian hormone signaling pathway." Mol Hum Reprod 22(9): 669-678.

9.Jung, D. and K. Kee* (2015). "Insights into female germ cell biology: from in vivo development to in vitro derivations." Asian J Androl 17(3): 415-420.

10.Liu, C., J. Lin, L. Li, Y. Zhang, W. Chen, Z. Cao, H. Zuo, C. Chen* and K. Kee* (2015). "HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells." Sci Rep 5: 7653.

11.Lin, J., H. Chen, L. Luo, Y. Lai, W. Xie and K. Kee* (2015). "Creating a monomeric endonuclease TALE-I-SceI with high specificity and low genotoxicity in human cells." Nucleic Acids Res 43(2): 1112-1122.

12.Chung, J., R. Dash, K. Kee, J. K. Barral, H. Kosuge, R. C. Robbins, D. Nishimura, R. A. Reijo-Pera and P. C. Yang* (2012). "Theranostic effect of serial manganese-enhanced magnetic resonance imaging of human embryonic stem cell derived teratoma." Magn Reson Med 68(2): 595-599.

13.Nguyen, H. N., B. Byers, B. Cord, A. Shcheglovitov, J. Byrne, P. Gujar, K. Kee, B. Schule, R. E. Dolmetsch, W. Langston, T. D. Palmer* and R. R. Pera* (2011). "LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress." Cell Stem Cell 8(3): 267-280.

14.Panula, S., J. V. Medrano, K. Kee, R. Bergstrom, H. N. Nguyen, B. Byers, K. D. Wilson, J. C. Wu, C. Simon, O. Hovatta and R. A. Reijo Pera* (2011). "Human germ cell differentiation from fetal- and adult-derived induced pluripotent stem cells." Hum Mol Genet 20(4): 752-762.

15.Kee, K. *, M. Flores, M. I. Cedars and R. A. Reijo Pera* (2010). "Human primordial germ cell formation is diminished by exposure to environmental toxicants acting through the AHR signaling pathway." Toxicol Sci 117(1): 218-224.

16.Kee, K., R. A. Pera and P. J. Turek* (2010). "Testicular germline stem cells." Nat Rev Urol 7(2): 94-100.

17.Kee, K., V. T. Angeles, M. Flores, H. N. Nguyen and R. A. Reijo Pera* (2009). "Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation." Nature 462(7270): 222-225.

18.McElroy, S. L., K. Kee, N. Tran, J. Menses, L. C. Giudice and R. A. Reijo Pera* (2008). "Developmental competence of immature and failed/abnormally fertilized human oocytes in nuclear transfer." Reprod Biomed Online 16(5): 684-693.

19.Kee, K. and R. A. Reijo Pera* (2008). "Human germ cell lineage differentiation from embryonic stem cells." CSH Protoc 2008: pdb prot5048.

20.International Stem Cell Initiative (2007). "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative." Nat Biotechnol 25(7): 803-816.

21.Kee, K., J. M. Gonsalves, A. T. Clark and R. A. Pera* (2006). "Bone morphogenetic proteins induce germ cell differentiation from human embryonic stem cells." Stem Cells Dev 15(6): 831-837.

22.Henderson, K. A., K. Kee, S. Maleki, P. A. Santini and S. Keeney* (2006). "Cyclin-dependent kinase directly regulates initiation of meiotic recombination." Cell 125(7): 1321-1332.

23.Kee, K., R. U. Protacio, C. Arora and S. Keeney* (2004). "Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes." EMBO J 23(8): 1815-1824.

24.Arora, C., K. Kee, S. Maleki and S. Keeney* (2004). "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism." Mol Cell 13(4): 549-559.

25.Kee, K. and S. Keeney* (2002). "Functional interactions between SPO11 and REC102 during initiation of meiotic recombination in Saccharomyces cerevisiae." Genetics 160(1): 111-122.

26.Kee, K., L. Niu and E. Henderson* (1998). "A Tetrahymena thermophila G4-DNA binding protein with dihydrolipoamide dehydrogenase activity." Biochemistry 37(12): 4224-4234.




联系方式

Email: kkee at tsinghua.edu.cn

Phone: +86-10-62794200

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