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基础医学系

倪建泉

履历

    2010年10月-现在,清华大学医学院研究员,博导

    2009年1月-2010年9月,博后,哈佛大学医学院遗传系,霍华德休斯医学研究所,波士顿,美国

    2007年1月-2008年12月,助理,JFRC,霍华德休斯医学研究所,华盛顿,美国

    2006年5月-2006年12月,博后,哈佛大学医学院遗传系,霍华德休斯医学研究所,波士顿,美国

    2001年10月-2006年4月,博士,FMI,巴塞尔大学,巴塞尔,瑞士



研究领域与方向

干细胞与表观遗传学

在过去的几年中,我的实验室专注于两个主要的研究方向:第一,我们利用果蝇模型研究基因表达和干细胞维持的表观遗传机制,取得了一系列成果。第二,我们开发了新的转基因干扰技术和基于CRISPR/Cas9系统的基因组编辑技术,为整个果蝇研究领域做出了重大贡献。将来,我们将继续探索干细胞增殖与分化的分子调控机制,着重关注染色质在其中的调控作用。此外,我们目前在细胞免疫学及癌症研究方面已经取得可观的前期结果,将继续深入研究相关分子机制。我相信,科学研究没有捷径,一分耕耘,一分收获

1. 干细胞增殖与分化机制

2. 细胞信号的调控与协同

3. 细胞信号与表观遗传学

4. 分子和遗传工具的研发前期结果,将继续深入研究相关分子机制。

实验室网站:
 http://fly.redbux.cn/


学术荣誉与奖励

关键词

干细胞, 细胞信号转导, 表观遗传学

代表性论文

1.Yu Jia, Rong-Gang Xu, Xingjie Ren, Ben Ewen-Campen, Rajendhran Rajakumar, Jonathan Zirin, Donghui Yang-Zhou, Ruibao Zhao, Fang Wang, Decai Mao, Ping Peng, Huan-Huan Qiao, Xia Wang, Lu-Ping Liu, Bowen Xu, Jun-Yuan Ji, Qingfei Lu, Jin Sun, Norbert Perrimon*, Jian-Quan Ni*.Next generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM. Proc Natl Acad Sci U S A, accepted.      

2.Ben Ewen-Campen, Donghui Yang-Zhou, Vitoria R. Fernandes, Delfina P. Gonzalez, Lu-Ping Liu, Rong Tao, Xingjie Ren, Jin Sun, Yanhui Hu, Jonathan Zirin, Stephanie E. Mohr, Jian-Quan Ni*,  Norbert Perrimon*. Optimized strategy for in vivo Cas9-activation in Drosophila. Proc Natl Acad Sci U S A, 2017, 114(35):9409-9414.    

3.Xingjie Ren, Kristof Holsteens, Haiyi Li, Jin Sun, Yifan Zhang, Lu-Ping Liu, Qingfei Liu* & Jian-Quan Ni*. Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system. Science China Life Sciences, 2017, 60(5):476-489.     

4.Zhihao Yang, Jin Sun, Yuzhao Hu, Fang Wang, Xia Wang, Huan-Huan Qiao, Jiang Xu, Decai Mao, Xingjie Ren, Li-Xia Pan, Rong-Gang Xu, Bo-Wen Xu, Yifan Zhang, Haiyi Li, Wei Miao, Yanhui Hu, Zhijie Chang, Dong Wang, Haitao Li, Zai Chang, Lu-Ping Liu, Qingfei Liu, Jian-Quan Ni*. Histone H1 defect in escort cells triggers germline tumor in Drosophila ovary. Developmental Biology, 2017, 424(1):40-49.

5.Tinglin Lu, Su Wang, Yuan Gao, Ying Mao, Zhihao Yang, Luping Liu, Xiaoqing Song, Jianquan Ni* and Ting Xie*. COP9-Hedgehog Axis Regulates the Function of the Germline Stem Cell Progeny Differentiation Niche in the Drosophila Ovary, Development. 2015

6. Jin Sun, Hui-Min Wei, Jiang Xu, Jian-Feng Chang, Zhihao Yang, Xingjie Ren, Wen-Wen Lv, Lu-Ping Liu, Li-Xia Pan, Xia Wang, Huan-Huan Qiao, Bing Zhu, Jun-Yuan Ji, Dong Yan, Ting Xie, Fang-Lin Sun*, Jian-Quan Ni*. Histone H1-Mediated Epigenetic Regulation Controls Germline Stem Cell Self-Renewal by Modulating H4K16 Acetylation, Nature Communications. 2015 Nov 19;6:8856. doi: 10.1038/ncomms9856.

7. Li-Xia Pan, Wenbing Xie, Kai-Le Li, Zhihao Yang, Jiang Xu, Wenhao Zhang, Lu-Ping Liu, Xingjie Ren, Zhimin He, Junyu Wu, Jin Sun, Hui-Min Wei, Da-Liang Wang, Wei Xie, Wei Li, Jian-Quan Ni*, and Fang-Lin Sun*. Heterochromatin remodeling by CDK12 contributes to learning in Drosophila, Proc Natl Acad Sci USA. 2015 Nov 10;112(45):13988-93. doi: 10.1073/pnas.1502943112. Epub 2015 Oct 27.

8. Jiang Xu, Xingjie Ren, Jin Sun, Xia Wang, Huan-Huan Qiao, Bo-Wen Xu, Lu-Ping Liu, Jian-Quan Ni*, A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila, J Genet Genomics. 2015 Apr 20;42(4):141-9.

9. Xingjie Ren, Zhihao Yang, Jiang Xu, Jin Sun, Decai Mao, Su-Juan Yang, Huan-Huan Qiao, Xia Wang, Qun Hu, Patricia Deng, Yanhui Hu, Lu-Ping Liu, Jun-Yuan Ji, Jin Billy Li*, Jian-Quan Ni*, Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila. Cell Reports. 2014 Nov 6;9(3):1151-62.

10. Xingjie Ren, Zhihao Yang, Decai Mao, Zai Chang, Huan-Huan Qiao, Xia Wang, Jin Sun, Qun Hu, Yan Cui, Lu-Ping Liu, Jun-Yuan Ji, Jiang Xu*, Jian-Quan Ni*. Performance of the Cas9 Nickase System in Drosophila melanogaster. G3 (Bethesda). 2014 Aug 15;4(10):1955-62.

11. Lei Pan, Su Wang, Tinglin Lu, Changjiang Weng, Xiaoqing Song, Joseph K. Park, Jin Sun, Zhi-Hao Yang, Junjing Yu, Hong Tang, Dennis M. McKearin, Daniel A. Chamovitz, Jianquan Ni, Ting Xie. Protein competition switches the function of COP9 from self-renewal to differentiation. Nature. 2014 Oct 9;514(7521):233-6.

12. Xingjie Ren, Jin Sun, Benjamin E. Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu, Zhihao Yang, Decai Mao, Lingzhu Sun, Qujie Wu, Jun-Yuan Ji, Jianzhong Xi, Stephanie E. Mohr, Jiang Xu*, Norbert Perrimon*, Jian-Quan Ni*. An optimized gene editing technology for Drosophila melanogaster using germline-specific Cas9, Proc Natl Acad Sci USA. 2013 Nov 19;110(47):19012-7.

13. Jian-Quan Ni, Rui Zhou, Benjamin Czech, Lu-Ping Liu, Lizabeth Perkins, Gregory Hannon and Norbert Perrimon. A genome-scale shRNA resource for transgenic RNAi in Drosophila . Nature Methods. 2011 May;8(5):405-7.

14. Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nature Methods. 2008 January; 5(1): 49–51.


联系方式

Email: nijq at tsinghua.edu.cn

Phone: +86-10-62789396

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